Sol-gel immobilization of acetylcholinesterase for the determination of organophosphate pesticides in olive oil with biosensors
Identifieur interne : 000024 ( Maroc/Analysis ); précédent : 000023; suivant : 000025Sol-gel immobilization of acetylcholinesterase for the determination of organophosphate pesticides in olive oil with biosensors
Auteurs : Najwa Ben Oujji [France, Maroc] ; Idriss Bakas [France, Maroc] ; Georges Istamboulie [France] ; Ihya Ait-Ichou [Maroc] ; Elhabib Ait-Addi [Maroc] ; Regis Rouillon [France] ; Thierry Noguer [France]Source :
- Food control [ 0956-7135 ] ; 2013.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Pesticide, Insecticide.
English descriptors
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Abstract
This paper presents the construction of amperometric biosensors for the detection of organophosphorus insecticides widely used in the treatment of olive trees. The systems are based on the immobilisation of acetylcholinesterase on screen-printed electrodes by bioencapsulation in a sol-gel composite. The enzyme activity was estimated by measuring the thiocholine produced by the enzymatic hydrolysis of the acetylthiocholine chloride using cobalt phtalocyanine as mediator. The developed devices have been used to carry out inhibition studies with three pesticides: malathion, methidathion and dimethoate (in their oxidized form), and tested using standard solutions and real samples of olive oil. These biosensors showed good operational stability as they maintained their initial analytical signal response during 10 successive measurements, and a good reproducibility with a relative standard deviation of 3%. The limits of detection of the developed devices were very compatible with the maximum residue limit tolerated by international regulations, they were as low as 10-9 M for the widely used pesticide malaoxon(oxidized malathion). The developed sensors were successfully used for the determinations of insecticides in real samples of olive oil.
Affiliations:
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Environment Team, Chemistry Department, Faculty of Science, BP 8106 Cité Dakhla</s1><s2>Agadir</s2><s3>MAR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Maroc</country><wicri:noRegion>Agadir</wicri:noRegion></affiliation></author><author><name sortKey="Istamboulie, Georges" sort="Istamboulie, Georges" uniqKey="Istamboulie G" first="Georges" last="Istamboulie">Georges Istamboulie</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>University of Perpignan, IMAGES EA 4218, Building S 52 Av Paul Alduy</s1><s2>66860 Perpignan</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName></affiliation></author><author><name sortKey="Ait Ichou, Ihya" sort="Ait Ichou, Ihya" uniqKey="Ait Ichou I" first="Ihya" last="Ait-Ichou">Ihya Ait-Ichou</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>University Ibn Zohr, AQUAMAR, Photocatalysis and Environment Team, Chemistry Department, Faculty of Science, BP 8106 Cité Dakhla</s1><s2>Agadir</s2><s3>MAR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Maroc</country><wicri:noRegion>Agadir</wicri:noRegion></affiliation></author><author><name sortKey="Ait Addi, Elhabib" sort="Ait Addi, Elhabib" uniqKey="Ait Addi E" first="Elhabib" last="Ait-Addi">Elhabib Ait-Addi</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>University Ibn Zohr, AQUAMAR, Photocatalysis and Environment Team, Chemistry Department, Faculty of Science, BP 8106 Cité Dakhla</s1><s2>Agadir</s2><s3>MAR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Maroc</country><wicri:noRegion>Agadir</wicri:noRegion></affiliation></author><author><name sortKey="Rouillon, Regis" sort="Rouillon, Regis" uniqKey="Rouillon R" first="Regis" last="Rouillon">Regis Rouillon</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>University of Perpignan, IMAGES EA 4218, Building S 52 Av Paul Alduy</s1><s2>66860 Perpignan</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName></affiliation></author><author><name sortKey="Noguer, Thierry" sort="Noguer, Thierry" uniqKey="Noguer T" first="Thierry" last="Noguer">Thierry Noguer</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>University of Perpignan, IMAGES EA 4218, Building S 52 Av Paul Alduy</s1><s2>66860 Perpignan</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Perpignan</settlement></placeName></affiliation></author></analytic><series><title level="j" type="main">Food control</title><title level="j" type="abbreviated">Food control</title><idno type="ISSN">0956-7135</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Food control</title><title level="j" type="abbreviated">Food control</title><idno type="ISSN">0956-7135</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amperometry</term><term>Biosensor</term><term>Eel</term><term>Electrodes</term><term>Immobilization</term><term>Insecticide</term><term>Olive oil</term><term>Organophosphorus compounds</term><term>Pesticides</term><term>Sol gel process</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Procédé sol gel</term><term>Immobilisation</term><term>Organophosphoré</term><term>Pesticide</term><term>Huile olive</term><term>Biodétecteur</term><term>Anguille</term><term>Insecticide</term><term>Ampérométrie</term><term>Electrode</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Pesticide</term><term>Insecticide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This paper presents the construction of amperometric biosensors for the detection of organophosphorus insecticides widely used in the treatment of olive trees. The systems are based on the immobilisation of acetylcholinesterase on screen-printed electrodes by bioencapsulation in a sol-gel composite. The enzyme activity was estimated by measuring the thiocholine produced by the enzymatic hydrolysis of the acetylthiocholine chloride using cobalt phtalocyanine as mediator. The developed devices have been used to carry out inhibition studies with three pesticides: malathion, methidathion and dimethoate (in their oxidized form), and tested using standard solutions and real samples of olive oil. These biosensors showed good operational stability as they maintained their initial analytical signal response during 10 successive measurements, and a good reproducibility with a relative standard deviation of 3%. The limits of detection of the developed devices were very compatible with the maximum residue limit tolerated by international regulations, they were as low as 10<sup>-9</sup> M for the widely used pesticide malaoxon(oxidized malathion). The developed sensors were successfully used for the determinations of insecticides in real samples of olive oil.</div></front></TEI><affiliations><list><country><li>France</li><li>Maroc</li></country><region><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li></region><settlement><li>Perpignan</li></settlement></list><tree><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Oujji, Najwa Ben" sort="Oujji, Najwa Ben" uniqKey="Oujji N" first="Najwa Ben" last="Oujji">Najwa Ben Oujji</name></region><name sortKey="Bakas, Idriss" sort="Bakas, Idriss" uniqKey="Bakas I" first="Idriss" last="Bakas">Idriss Bakas</name><name sortKey="Istamboulie, Georges" sort="Istamboulie, Georges" uniqKey="Istamboulie G" first="Georges" last="Istamboulie">Georges Istamboulie</name><name sortKey="Noguer, Thierry" sort="Noguer, Thierry" uniqKey="Noguer T" first="Thierry" last="Noguer">Thierry Noguer</name><name sortKey="Rouillon, Regis" sort="Rouillon, Regis" uniqKey="Rouillon R" first="Regis" last="Rouillon">Regis Rouillon</name></country><country name="Maroc"><noRegion><name sortKey="Oujji, Najwa Ben" sort="Oujji, Najwa Ben" uniqKey="Oujji N" first="Najwa Ben" last="Oujji">Najwa Ben Oujji</name></noRegion><name sortKey="Ait Addi, Elhabib" sort="Ait Addi, Elhabib" uniqKey="Ait Addi E" first="Elhabib" last="Ait-Addi">Elhabib Ait-Addi</name><name sortKey="Ait Ichou, Ihya" sort="Ait Ichou, Ihya" uniqKey="Ait Ichou I" first="Ihya" last="Ait-Ichou">Ihya Ait-Ichou</name><name sortKey="Bakas, Idriss" sort="Bakas, Idriss" uniqKey="Bakas I" first="Idriss" last="Bakas">Idriss Bakas</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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